Out of your sealer, most will tell you to use 600.. You could use 400 for solids, and 600 for metallics and pearls, but 600 is always safe bets for anyone..
I leave my last coat of 2k in 320 or epoxy which ever Im using, then I use the old 1:1:1 epoxy as my sealer and spray base 30 minutes later.. Generally its soo thin that trash isnt an issue.. When I have had some issue with trash in that thin of a coat, I would use a grey scuff pad, and base right over it.. However, I have had a time or 2 where a silver came back and bit me in the butt using the grey scuff pad, so I always recommend the 600..
There are allot of instances where you can use certain things at certain times, but not always and not all colors etc.. So just stick with the 600
Happy Spraying